Recombinant Truncated Flagellin of Burkholderia pseudomallei as a Molecular Probe for Diagnosis of Melioidosis
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چکیده
منابع مشابه
Recombinant truncated flagellin of Burkholderia pseudomallei as a molecular probe for diagnosis of melioidosis.
Current serological tests for melioidosis, using impure or uncharacterized cell antigens from Burkholderia pseudomallei, have problems in detection sensitivity and specificity. Therefore, we designed and expressed the recombinant flagellin (truncated at both the N- and C-terminal ends), and used the antigen to develop an indirect enzyme-linked immunosorbent assay (ELISA) to diagnose melioidosis...
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Burkholderia pseudomallei, the etiologic agent of human melioidosis, is capable of causing severe acute infection with overwhelming septicemia leading to death. A high rate of recurrent disease occurs in adult patients, most often due to recrudescence of the initial infecting strain. Pathogen persistence and evolution during such relapsing infections are not well understood. Bacterial cells pre...
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The genus Burkholderia is currently composed of many species, but only three are notable pathogens for humans or animals: the former cepacia complex (described in Chapter 220) pseudomallei (the agent of melioidosis), and mallei (the agent of equine glanders). All three are aerobic, nonsporulating, straight or slightly curved gram-negative bacilli that were formerly placed in the genus Pseudomonas.
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Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chrom...
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Yao-Shen Chen, Ssu-Ching Chen, Tsu-Rong Wu, Chih-Ming Kao and Ya-Lei Chen* Section of Infectious Disease, Kaohsiung Veterans General Hospital, Graduate Institute of Environmental Education and Department of Biotechnology, National Kaohsiung Normal University, Department of Medical Technology, Fooyin Unversity, Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung, T...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2003
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cdli.10.3.423-425.2003